Journal: Cell Communication and Signaling : CCS

Article Title: CDK7, CDK9, or CDK11 inhibition reduces neutrophil-driven inflammation and tissue damage in experimental autoimmune models

doi: 10.1186/s12964-026-03219-x

Figure Lengend Snippet: Systemic and topical CDK7i, CDK9i, and CDK11i treatment reduces disease severity in local EBA. A EBA was induced by injection of anti-mCOL7 c IgG and the mice were treated with CDKi either systemically or topically. B Exemplary clinical and histological images are shown with the scale bars corresponding to 100 µM. Asterisks indicate cell infiltrates and arrows indicate binding of C3/IgG to the dermal-epidermal junction. C The affected ear surface area (AESA) and the H&E score were evaluated in the systemic treatment group for MC180295 , ( D ) THZ2, ( E ) THZ1 2HCl, and ( F ) OTS964. G Stainings for Ly6G and F4/80 were performed on ear sections from mice systemically treated with MC180295 and control mice. The scale bar refers to 100 μm. Ly6G or F4/80 are depicted in red, DAPI in blue. H For the topical model, AESA was analyzed as well. Two-way ANOVA with Šidák’s multiple comparisons test was performed for AESA evaluation, except for the systemic treatment with THZ2 and MC180295 a mixed-effects analysis with Šidák’s multiple comparisons test was conducted. For H&E scores and stainings, Mann-Whitney test was carried out. * p ≤ 0.05. ** p ≤ 0.01. *** ≤ p 0.001. **** p ≤ 0.0001. n = 8

Article Snippet: Paraffin-embedded ear sections of MC180295 -treated and control antibody transfer-induced EBA mice or paw sections of KBxN STA mice were subjected to immunofluorescence staining for Ly6G or F4/80 as described previously [ ] and mounted with DAPI Fluoromount-G (Southern Biotech, Birmingham, Alabama, USA).

Techniques: Injection, Binding Assay, Control, Analysis, MANN-WHITNEY

Journal: Cell Communication and Signaling : CCS

Article Title: CDK7, CDK9, or CDK11 inhibition reduces neutrophil-driven inflammation and tissue damage in experimental autoimmune models

doi: 10.1186/s12964-026-03219-x

Figure Lengend Snippet: CDK9 inhibition with MC180295 improves disease severity in KBxN STA, but not in ITP. A To induce KBxN STA, mice were injected with arthritic KBxN serum and treated with MC180295 for seven days. B The course of the disease score is depicted and ( C ) reflected by the clinical presentation of the paws and the histological stainings. The scale bars represent 100 μm. Asterisks indicate proteoglycan staining (Safranin-O) or cell infiltration (H&E). D Cytokine levels were measured in serum and significantly changed cytokines are shown. E Stainings for Ly6G and F4/80 were performed on paw sections from mice systemically treated with MC180295 and control mice. The scale bar refers to 100 μm. Ly6G or F4/80 are depicted in red, DAPI in blue. F ITP was induced by anti-platelet IgG2c-induced platelet depletion and MC180295 was applied at 24 h and 4 h before depletion. G Absolute and ( H ) relative platelet counts were monitored for up to 24 h and values normalized to 100% at 0 h are indicated. B , G , H For arthritis score and platelet counts, two-way ANOVA with Šidák’s multiple comparisons test was performed. (D, E) For cytokine levels and stainings, Mann-Whitey test was conducted. * p ≤ 0.05. ** p ≤ 0.01. **** p ≤ 0.0001. (A-E) n = 5 (treatment) and n = 4 (control). (F-H) n = 4

Article Snippet: Paraffin-embedded ear sections of MC180295 -treated and control antibody transfer-induced EBA mice or paw sections of KBxN STA mice were subjected to immunofluorescence staining for Ly6G or F4/80 as described previously [ ] and mounted with DAPI Fluoromount-G (Southern Biotech, Birmingham, Alabama, USA).

Techniques: Inhibition, Injection, Staining, Control